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| 湘西地区蛇足石杉内生真菌的分离 | |
| 引用本文： | 徐巧玉,何兴兵,唐克华,唐源江,李菁,田兴军.湘西地区蛇足石杉内生真菌的分离[J].生命科学研究,2008,12(4). |
| 作者姓名： | 徐巧玉 何兴兵 唐克华 唐源江 李菁 田兴军 |
| 作者单位： | 1. 南京大学,生命科学学院,中国江苏,南京,210093 2. 吉首大学,城乡资源与规划学院,中国湖南,张家界,427000;吉首大学-武陵山区植物资源保护与利用湖南省高校重点实验室,中国湖南,吉首,416000 3. 中国科学院华南植物园,中国广东,广州,510650 4. 吉首大学-武陵山区植物资源保护与利用湖南省高校重点实验室,中国湖南,吉首,416000 |
| 基金项目： | 湖南省自然科学基金项目(2007NK3075):广东省科技计划资助项目 |
| 摘 要： | 采集不同生境的药用植物蛇足石杉,运用不同灭菌方法处理外植体,并用PDA平板培养基及孟加拉红培养基进行内生真菌的分离纯化.经过几种灭菌方法的摸索和比较研究,发现蛇足石杉外植体的灭菌十分困难(尤其是其茎部),难以在保持植物鲜活状态下达到一次彻底灭菌,有必要二次灭菌.通过二次灭菌分离得到的9株内生真菌中,来自蛇足石杉茎部的6株,来自叶片的3株.蛇足石杉的9株内生真菌在平板培养时生长极其缓慢,仅一株可在培养时产生孢子. |
| 关 键 词： | 蛇足石杉 内生真菌 灭菌 分离 |
Isolation of Endophytic Fungi from Huperzia Serrata in Western Hunan Province |
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| XU Qiao-yu,HE Xing-bing,TANG Ke-hua,TANG Yuan-jiang,LI Jing,TIAN Xing-jun.Isolation of Endophytic Fungi from Huperzia Serrata in Western Hunan Province[J].Life Science Research,2008,12(4). | |
| Authors: | XU Qiao-yu HE Xing-bing TANG Ke-hua TANG Yuan-jiang LI Jing TIAN Xing-jun |
| Institution: | XU Qiao-yu1,HE Xing-bing2,3,TANG Ke-hua2,3,TANG Yuan-jiang4,LI Jing3*,TIAN Xing-jun1 |
| Abstract: | Huperzia serrata was collected from different environment for the isolation of endophytic fungi and finding the effective sterilizing method. The result showed that the epiphytic fungi were difficult to be sterilized thoroughly for one time sterilization,and the second time sterilization was needed,especially for the stem. Of nine endophytic fungi strains isolated from healthy H. serrata with conventional isolation method,6 strains were from stems and 3 strains were from leaves. The isolated fungi grew slowly on culture medium,and only one strain was observed to have spores. |
| Keywords: | Huperzia serrata(Thunb )Trev endophytic fungi sterilizing isolation |
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