| 人参植物与皂苷生物合成相关的差减cDNA文库构建及基因差异表达分析 |
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| 引用本文： | 罗志勇,刘水平,陆秋恒,陈湘晖,文斌,罗建清,胡维新.人参植物与皂苷生物合成相关的差减cDNA文库构建及基因差异表达分析[J].生命科学研究,2003,7(4):324-328. |
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| 作者姓名： | 罗志勇 刘水平 陆秋恒 陈湘晖 文斌 罗建清 胡维新 |
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| 作者单位： | 1. 中南大学,湘雅医学院,生物科学与技术学院,分子生物学研究所,中国湖南,长沙,410078
2. 中南大学,湘雅医院,中国湖南,长沙,410078 |
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| 基金项目： | 国家自然科学基金资助项目(39800190)，湖南省自然科学基金资助项目(02JJY2045)，湖南医科大学杰出青年科学基金资助项目(200002)，湖南省高校青年骨干教师基金资助项目(湘教通204号) |
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| 摘 要： | 应用抑制差减杂交技术，分别以源于4年和1年生人参根组织cDNA群体作为检测子(tester)与驱赶子(driver)，成功构建了与人参植物皂苷生物合成相关的差减cDNA文库，并时从中筛选的阳性cDNA克隆进行DNA测序及其序列分析、PCR及Northern印迹杂交鉴定．结果显示，获得的13个克隆为新基因序列．其中6个差减克隆系人参植物根生长发育阶段差异表达基因．目前，6个差异表达新基因的结构与功能仍在进一步研究中．
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| 关 键 词： | 人参植物 皂苷 生物合成 差减cDNA文库 基因差异表达 五加科 人参属 |
| 文章编号： | 1007-7847(2003)04-0324-05 |
| 修稿时间： | 2003年10月23 |
Construction of Subtractive cDNA Library and mRNA Differential Expression Analysis Involved in Ginsenoside Biosynthesis from Panax ginseng Root |
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| LUO Zhi-yong,LIU Shui-ping,LU Qiu-heng,CHEN Xiang-hui,WEN Bin,LUO Jian-qing,HU Wei-xin.Construction of Subtractive cDNA Library and mRNA Differential Expression Analysis Involved in Ginsenoside Biosynthesis from Panax ginseng Root[J].Life Science Research,2003,7(4):324-328. |
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| Authors: | LUO Zhi-yong LIU Shui-ping LU Qiu-heng CHEN Xiang-hui WEN Bin LUO Jian-qing HU Wei-xin |
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| Institution: | LUO Zhi-yong~1,LIU Shui-ping~1,LU Qiu-heng~1,CHEN Xiang-hui~2,WEN Bin~1,LUO Jian-qing~1,HU Wei-xin~1 |
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| Abstract: | Suppression Subtractive Hybridization (SSH) was utilized for the isolation of cDNA fragments for Panax ginseng root differentially expressed genes, and a subtracted cDNA library involved in ginsenoside biosynthesis from Panax ginseng roots was constructed. Forty cDNA clones selected randomly from the cDNA library were sequenced. Sequence information of the aboved clones was evaluated by Nucleotide Blast analysis. The results showed that nineteen cDNA clones represented the novel genes (ESTs), because no sequence homology with any known sequences was found in GenBank/DDBJ/EMBL databases. Six of all novel genes were differentially expressed in Panax ginseng roots and their structures and functions will be further studied. |
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| Keywords: | ginsenoside biosynthesis subtracted cDNA library suppression subtractive hybridization mRNA differential expression Panax ginseng root tissues |
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