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百合鳞片细胞形态发生中生理生化特性研究
引用本文:屈妹存,刘选明.百合鳞片细胞形态发生中生理生化特性研究[J].生命科学研究,1998,2(4):288-294.
作者姓名:屈妹存  刘选明
作者单位:湖南农业大学理学院
基金项目:湖南省科委自然科学基金
摘    要:四倍体百合鳞片外植体脱分化形成愈伤组织过程其蛋白质、核酸含量与蛋白酶、淀粉酶、过氧化物酶POD、酸性磷酸酯酶活性先升高后降低,淀粉与ATP含量呈降-升-降变化,SOD活性先降低后升高,愈伤组织分化芽过程,除ATP含量降低外,其余指标均不断升高.鳞片外植体直接分化发育不定芽过程中,仅见蛋白质、淀粉、ATP含量与SOD、酸性磷酸酯酶活性在5d前降低,其它时间各项指标均升高,直到不定芽发育完成再下降.器官发生型比器官型经历了更为深刻的内部生理生化变化.

关 键 词:百合,器官型,器官发生型,形态发生,生理生化

Studies on the Changes and Comparison of Physiology and Biochemistry in the Morphogenesis of Scale Explant of Lily in vitro
QU Shucun,LIU Xuanming,ZHOU,Puhua,LU Xiangyang,LUO,Zemin.Studies on the Changes and Comparison of Physiology and Biochemistry in the Morphogenesis of Scale Explant of Lily in vitro[J].Life Science Research,1998,2(4):288-294.
Authors:QU Shucun  LIU Xuanming  ZHOU  Puhua  LU Xiangyang  LUO  Zemin
Abstract:The results indicated:the content of protein,uncleic acid and the activity of protease,amylase,POD and acidic phosphatase increased before the callus formation,and the activity of SOD changed contrany to this in the same time,but the content of starch and ATP decreased in initial stage,then increased during the callus formation and decreased again after the callus formation.During the bud differentiation from calli,all targets increased except the ATP content.In organogeny type morphogenesis,the content of protein,starch,ATP and the activity of SOD,acidic phosphatase decreased only before the first five days,the other targets increased in the same time.During the period of the direct bud formation ,all targets increased.After the bud formation,those target all became to fall.The changes of physiology and biochemistry in cells were greater in organogeny type morphogenesis than in organ type morphogenesis.
Keywords:lily  organ  type  organogeny  type  morphogenesis  physiology  and biochemistry
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