从琼脂糖电泳凝胶中回收DNA的几种简便方法

介绍两类从普通琼脂糖电泳凝胶中回收ＤＮＡ的简便、快捷、高效且廉价的方法．第一类为电泳洗脱法．方法ａ：利用１．５ｍＬ微量离心管、ｌｍＬ吸头、尼龙网膜和透析膜做成的一个小装置，快速有效回ＤＮＡ，最终回收率为７０％左右．方法ｂ：不用ＤＥＡＥ－纤维素膜，而用透析膜在凝胶中作出横隔挡在ＤＮＡ条带前，最终回收率为５０％左右；第二类为冰冻融解法，最终回收率也在５０％左右．如果联合使用冰冻融解法和电泳洗脱法，回收率可进一步提高至９０％．

Several Convenient Methods of Recovering DNA from Agarose Electrophoretic Gels

Two categories of easy, rapid, effective and cheap methods for recovering DNA from agarose gel electrophoretic gels are reported. The first category is electroelution.Method a: A recovering unit was made by using 1.5 mL microcentrifuge tube, l mL tip, nylon web membrane and dialysis membrane.The final recovery rate of this rapid and effective method could be raised to 70%. Method b: A slit was cut in the gel immediately ahead of the DNA fragment(s) of interest and a sliver of dialysis membrane, instead of DEAE cellulose membrane,was inserted into the slit.The recovery rate of this electroelution method was about 50%.The second category is the freezingthawing method. It's the easiest way to obtain recovery rate of about 50%. Combination of the freezingthawing method and electroelution can increase the recovery rate to 90%.