| DNAJC10基因在鼻咽癌中的表达及表达下调机制的研究 |
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| 引用本文： | 周亮,任彩萍,单文姣,姚开泰.DNAJC10基因在鼻咽癌中的表达及表达下调机制的研究[J].生命科学研究,2006,10(4):362-366. |
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| 作者姓名： | 周亮 任彩萍 单文姣 姚开泰 |
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| 作者单位： | 中南大学,肿瘤研究所,中国湖南,长沙,410078 |
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| 基金项目： | 国家科技部恶性肿瘤发生与发展的基础性研究973项目(G1998051201) |
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| 摘 要： | 运用RT-PCR检测候选抑瘤基因DNAJC10在鼻咽癌组织中的表达,运用甲基化特异性PCR(MSPCR)技术、LOH和测序等技术分别检测DNAJC10基因在鼻咽癌组织中的甲基化状况、LOH和启动子突变情况.结果表明,DNAJC10基因在肿瘤组织中较对照慢性鼻咽炎组织表达明显下调(P<0.05).DNAJC10在鼻咽癌中不表现高甲基化,其LOH的缺失率为6.25%,突变率为66.7%.因此,DNAJC10基因的表达下调主要是其遗传改变(LOH和突变)所致.
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| 关 键 词： | DNAJC10基因 表达 甲基化 LOH 突变 鼻咽癌 |
| 文章编号： | 1007-7847(2006)04-0362-05 |
| 收稿时间： | 2006-09-14 |
| 修稿时间： | 2006-10-30 |
Studies on Expression and Regulation Mechanism of DNAJC10 Gene in NPC |
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| ZHOU Liang,REN Cai-ping,SHAN Wen-jiao,YAO Kai-tai.Studies on Expression and Regulation Mechanism of DNAJC10 Gene in NPC[J].Life Science Research,2006,10(4):362-366. |
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| Authors: | ZHOU Liang REN Cai-ping SHAN Wen-jiao YAO Kai-tai |
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| Institution: | Cancer Research Institute, Central South University, Changsha 410078, YAO Kai-tai Hunan, China |
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| Abstract: | In order to detect the expression level and regulation mechanism of DNAJC10 gene in nasopharyngeal carcinoma(NPC),the expression state,methylation status,loss of heterozygosity(LOH) and mutation of DNAJC10 gene were examined by RT-PCR,MSPCR,LOH assay and sequencing respectively.The results indicated DNAJC10 gene had a lower expression level in NPC tissues compared with that in chronic nasopharyngitis tissues by statistical analysis(P< 0.05),but hypermethylation of DNAJC10 was showed neither in NPC nor in chronic nasopharyngitis tissues.LOH and sequencing results showed DNAJC10 was found to have LOH and mutations in 6.25% and 66.7% of NPC biopsies,respectively.In conclusion,the results suggest down-regulation of DNAJC10 gene in NPC should be attributed to LOH and promoter mutation. |
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| Keywords: | DNAJC10 gene expression methylation LOH mutation NPC |
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