| 非特异性DNA的催化活性(Ⅰ)──应用酚试剂及FeCl_3检测DNA的酯酶活性 |
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| 引用本文： | 王身立,石东乔,刘文珍.非特异性DNA的催化活性(Ⅰ)──应用酚试剂及FeCl_3检测DNA的酯酶活性[J].生命科学研究,1998(1). |
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| 作者姓名： | 王身立 石东乔 刘文珍 |
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| 作者单位： | 湖南师范大学生物学系 |
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| 基金项目： | 湖南省科委与省教委资助 |
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| 摘 要： | 分别应用酚试剂与ＦｅＣｌ３来检测萘酚，表明ＤＮＡ能催化乙酸萘酯的水解，印证了以前关于ＤＮＡ具有酯酶活性的结论．ＦｅＣｌ３法可以作为检测ＤＮＡ酯酶活性的一种定量方法．
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| 关 键 词： | DNA，酯酶活性，酚试剂，三氯化铁 |
Determination of the Esterase like Activity of DNA by Using Phenol Reagent and FeCl 3 |
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| Wang,Shenli,Shi,Dongqiao,Liu,Wenzhen.Determination of the Esterase like Activity of DNA by Using Phenol Reagent and FeCl 3[J].Life Science Research,1998(1). |
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| Authors: | Wang Shenli Shi Dongqiao Liu Wenzhen |
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| Abstract: | By using phenol reagent and FeCl 3 respectively to determinate naphthyl phenol, it showed that naphthyl acetate could be hydrolyzed by DNA. The results corroborated the former conclusion that DNA had esterase like activity. |
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| Keywords: | DNA esterase like activity phenol reagent FeCl 3 |
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