| 红车轴草提取物对胃癌BGC-823细胞凋亡的影响 |
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| 引用本文： | 郑杰,刘艳平,宗永立,李忠魁,苏文,曾建国.红车轴草提取物对胃癌BGC-823细胞凋亡的影响[J].生命科学研究,2006,10(2):178-182. |
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| 作者姓名： | 郑杰 刘艳平 宗永立 李忠魁 苏文 曾建国 |
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| 作者单位： | 1. 中南大学,生物科学与技术学院,细胞生物学系,中国,湖南,长沙,410013
2. 九汇现代中药有限责任公司,中国,湖南,长沙,410016 |
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| 摘 要： | 探讨红车轴草(TrifoliumpratenseL)提取物对胃癌细胞株BGC-823的抑制增殖效应及诱导凋亡作用.我们采用不同浓度(50、100、250、500、1000mg/L)红车轴草提取物处理BGC-823细胞,采用四甲基偶氮唑盐(MTT)法检测药物对细胞的抑制作用,倒置显微镜观察细胞的形态学改变;AO/EB染色,荧光显微镜观察细胞凋亡形态;采用DNALadder观察DNA的降解;应用流式细胞仪观察细胞凋亡过程中的细胞周期的变化和细胞凋亡.结果显示在红车轴草提取物的作用下,BGC-823细胞呈凋亡改变,DNA琼脂糖凝胶电泳呈典型的凋亡特征.细胞凋亡的同时,细胞周期阻滞于G2/M期.实验结果表明红车轴草提取物能抑制胃癌BGC-823细胞增殖,能诱导胃癌细胞凋亡.
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| 关 键 词： | 红车轴草提取物 胃癌细胞BGC-823 凋亡 增殖 |
| 文章编号： | 1007-7847(2006)02-0178-05 |
| 收稿时间： | 2005-12-11 |
| 修稿时间： | 2006-03-23 |
Effect of Trifoliumpratense L. Extract on Apoptosis of BGC-823 Cells |
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| ZHENG Jie,LIU Yan-ping,ZONG Yong-li,LI Zhong-kui,SU Wen,ZENG Jian-guo.Effect of Trifoliumpratense L. Extract on Apoptosis of BGC-823 Cells[J].Life Science Research,2006,10(2):178-182. |
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| Authors: | ZHENG Jie LIU Yan-ping ZONG Yong-li LI Zhong-kui SU Wen ZENG Jian-guo |
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| Institution: | 1. Cell Biology of Bioscience and Technology Academy, Central South University, Changsha 410013, Hunan, China; 2. Phytoway Inc, Changsha 410016, Hunan, China |
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| Abstract: | To investigate whether Trifoliumpratense L. extract induces apoptosis in gastric cancer cell line BGC-823.Cell proliferation was assessed by MTT assay.Morphological changes of apoptosis were observed with invert microscope apoptotic morphology was observed by staining the BGC-823 cell with AO/EB;DNA Ladder, cell cycle and apoptosis were examined by DNA agarose gel eletronphores and PI fluorescence flow cytometry (FCM),respectively.Result showed under the influence of Trifoliumpratense L. extract,BGC-823 cell exhibited changes of apoptosis both in morphology and in the characteristics of electrophoresis.Meanwhile the cell cycle also blocked in G2/M.The study indicated Trifoliumpratense L. extract could inhibit proliferation and induce apoptosis in gastric cancer BGC-823 cell. |
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| Keywords: | Trifoliumpratense L extract gastric cancer BGC-823 cell apoptosis proliferation |
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