| 利用番茄为受体表达轮状病毒外壳蛋白VP7的研究 |
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| 引用本文： | 沈文涛,周鹏.利用番茄为受体表达轮状病毒外壳蛋白VP7的研究[J].生命科学研究,2004,8(3):245-249. |
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| 作者姓名： | 沈文涛 周鹏 |
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| 作者单位： | 中国热带农业科学院,热带生物技术研究所,热带作物生物技术国家重点实验室,中国,海南,海口,571101 |
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| 基金项目： | 国家自然科学基金资助项目(30060033) |
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| 摘 要： | 将轮状病毒外壳蛋白VP7基因克隆到植物表达载体pBll21，并转化到根癌农杆菌(Agrobacterium tumefaciens)菌株EHAl05中，采用叶盘转化法转化番茄(1ycopersicon esculentumMill．)栽培品种TX0014，获得了转基因植株．经PCR、PCR—Southernblot和Southernblot分析表明：VP7基因已整合到转基因番茄植株的核基因组中．RT—PCR、Westernblot分析表明：VP7蛋白在叶片和果实中均获得了表达．
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| 关 键 词： | 轮状病毒 VP7 番茄 遗传转化 |
| 文章编号： | 1007-7847(2004)03-0245-05 |
| 修稿时间： | 2004年6月8日 |
Expression of VP7 of Rotavirus Outer Capsid Protein in Transgenic Tomato |
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| SHEN Wen-tao,ZHOU Peng.Expression of VP7 of Rotavirus Outer Capsid Protein in Transgenic Tomato[J].Life Science Research,2004,8(3):245-249. |
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| Authors: | SHEN Wen-tao ZHOU Peng |
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| Abstract: | Cotyledons of tomato were transform ed with Agrobacterium Tumefaciens EHA105carrying the gene VP7coding rotavirus outer capsid prote in.After several times kanamycin-r esistant selection,the regenerate d tomato plantlets were analyzed further by PCR,PCR-Southern blot and Sou thern blot,the results confirmed that the VP7gene were integrated into the genome .The results of RT-PCR and Western bl ot of transgenic plants showed that VP7gene was expressed in leaves and frui ts of transgenic plants. |
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| Keywords: | rotaviruses VP7 tomato genetic transformation |
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