Electrotransformation ofStreptococcus sanguis Challis |
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| Authors: | Dr George A Somkuti Dennis H Steinberg |
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| Institution: | (1) Eastern Regional Research Center, US Department of Agriculture, ARS, 600 East Mermaid Lane, 19118 Philadelphia, Pennsylvania, USA |
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| Abstract: | Plasmid DNA was introduced into noncompetent cells ofStreptococcus sanguis Challis by an electrotransformation technique. The procedure was simple and rapid, did not require elaborate pretreatment of cells, and yielded transformant colonies in 24 h. The maximum transformation efficiency attained was 2.1×104 transformants per  g of pVA736. Molecular rearrangements and deletions were not detected in plasmid DNA isolated from transformants. |
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