Chlamydial Antiapoptotic Activity Involves Activation of the Raf/MEK/ERK Survival Pathway |
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Authors: | Email author" target="_blank">Kun?DuEmail author Qun?Zheng Ming?Zhou LiSha?Zhu Biao?Ai Li?Zhou |
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Institution: | (1) Department of Clinical Laboratory, The First Affiliated Hospital of Yangtze University, Jingzhou, 434000, Hubei, China |
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Abstract: | Chlamydiae are obligate intracellular bacteria that cause variety of human diseases. Chlamydia-infected host cells are profoundly resistant to apoptosis induced by many different apoptotic stimuli. The inhibition of
apoptosis is thought to be an important immune escape mechanism allowing chlamydiae to productively complete their obligate
intracellular growth cycle. Infection with chlamydiae can activate the Raf/MEK/ERK pathway. Because the survival pathway can
modulate apoptosis, we used MEK-specific inhibitor U0126 and Raf-specific inhibitor GW5074 to examine the role of Raf/MEK/ERK
pathway in chlamydial antiapoptotic activity. Apoptosis was induced by staurosporine (STS) and detected by morphology, DNA
fragmentation, caspase-3 activation, and poly (ADP-ribose) polymerase cleavage. Inhibition of the pathway sensitized Chlamydia-infected cells to STS-mediated cell apoptosis. The data indicate that chlamydial antiapoptotic activity involves activation
of the Raf/MEK/ERK survival pathway. |
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