Molecular Detection of <Emphasis Type="Italic">Penicillium griseofulvum</Emphasis> as the Coastal Pollution Indicator |
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Authors: | Zhengping?Shi Shumeng?Bai Email author" target="_blank">Li?TianEmail author Huichao?Jiang Jiuming?Zhang |
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Institution: | (1) Qingdao University of Science & Technology, Qingdao, 266042, China;(2) First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061, China; |
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Abstract: | Molecular methods were carried out to detect Penicillium griseofulvum, a dominant species related to heavy metal pollution, which was screened from marine contaminated sediments. Based on differences
in internal transcribed spacer (ITS) sequences of Penicillium genus and specific isoamyl alcohol oxidase (IAO) sequences, species-specific primers AS1/RS4 and IAO1/IAO2 of Penicillium griseofulvum were designed and synthesized which were then employed in optimized PCR systems. The detection sensitivities were compared
through ordinary PCR and nested-PCR using two pairs of primers, respectively. Both primer pairs could exclusively amplify
destined DNA fragment from contaminated environmental samples in our researches. As for primers AS1/RS4, the detection sensitivity
for spores (pure spore DNA) could be 10 fg/μl and 10 spores, respectively, and the detection sensitivity for the sediments
was 102 spores/0.25 g sediments. While the detection sensitivity of IAO1/IAO2 primers was lower than that of AS1/RS4. Despite the
difference in detection sensitivity, it is feasible that the species-specific primers could be used as probes for the detection
of environmental pollution dominant species, Penicillium griseofulvum, since the frequency of occurrence and amount of this strain could preferably indicate the pollution degree. |
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