Isoelectric focusing patterns of staphylococcal exfoliative toxin |
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Authors: | Anna D Johnson-Winegar Leonard Spero |
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Institution: | (1) Department of Applied Toxin Research, U.S. Army Medical Research Institute of Infectious Diseases, 21701 Fort Detrick, Maryland, USA |
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Abstract: | Multiple components in two antigenic variants of staphylococcal exfoliative toxin were demonstrated by isoelectric focusing in polyacrylamide gel. The main components had an isoionic point of pH=7.0 while other components ranged from pI=4.5 to pI=8.5. Each individual component was shown to possess full biologic and serologic activity after focusing. Isoelectric focusing in the presence of 8 M urea also showed microheterogeneity in exfoliative toxin preparations. The various components appear to be reversible conformers, possibly raised by exposure to the ph gradient of the ampholines. |
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