Sulfatides Inhibit Binding of Helicobacter pylori to the Gastric Cancer Kato III Cell Line |
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Authors: | Torkel Wadström Siiri Hirmo Hajdi Novak Antonio Guzman Martina Ringnér-Pantzar Meeme Utt Pär Aleljung |
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Institution: | Department of Medical Microbiology, University of Lund, S?lvegatan 23, Lund S-22362, Sweden, SE
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Abstract: | Helicobacter pylori adhere to Kato III and Hela S3
cells in monolayer cultures. To explore whether cell surface glycoconjugates
on these two cell lines mediate binding of H. pylori, various
carbohydrates, glycoproteins, and glycolipids were tested to inhibit H.
pylori cell adhesion. The adhesion was measured (i) with a urease-based
assay and (ii) by cells stained with fluorescein. Sodium periodate and
sialidase treatment (but not α- or β-galactosidase, heparitinase,
lysozyme, or trypsin) inhibited H. pylori binding to both cell lines.
Sulfatides and sulfated glycoconjugates (50 μg/ml) but not heparin or a
number of simple carbohydrates inhibited binding (1 mg/ml). The two H.
pylori strains studied (CCUG 17874 and strain 25) showed high binding of
soluble 125I-labeled heparin and other sulfated carbohydrate
compounds.
Received: 5 July 1996 / Accepted: 17 October 1996 |
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