Differential Activity and Activation of Bacillus thuringiensis Insecticidal Proteins in Diamondback Moth, Plutella xylostella |
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| Authors: | Rose Monnerat Luke Masson Roland Brousseau Marianne Pusztai-Carey Dominique Bordat Roger Frutos |
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| Institution: | (1) Cenargen-EMBRAPA, SAIN-Parque Rural CP, 02372 CEP, 70770-970 Brasilia DF, Brazil , BR;(2) Institut de Recherche en Biotechnologie, CNRC, 6100 Avenue Royalmount, Montreal, Quebec, H4P 2R2, Canada , CA;(3) Case Western Reserve University, Cleveland, Ohio, USA , US;(4) CIRAD, Avenue Agropolis, B.P. 5035, 34032 Montpellier Cedex 1, France , FR |
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| Abstract: | Whole-crystal preparations from strains HD-1 and HD-133, activated Cry1Ab and Cry1C toxins as well as Cry1Aa, Cry1Ac, Cry1D,
and Cry2Aa protoxins were tested for toxicity to 2nd-instar larvae of the diamondback moth, Plutella xylostella. Mortality data recorded after 2 and 5 days provided different results that were related to differential rates of solubilization,
activation, and degradation of insecticidal crystal proteins. The two most active proteins are Cry1Ab and Cry1C, which are
both present in HD-133. The Cry1Ab protoxin is activated within 2 days, whereas activation of the Cry1C protoxin occurs between
2 and 5 days. HD-133 is more active than HD-1 immediately after infection and remains toxic over 5 days owing to the sequential
activation of its crystal components. Solubility properties of crystals and rates of activation of protoxins influence the
overall toxicity of HD-1 and HD-133 to the diamondback moth.
Received: 30 March 1999 / Accepted: 3 May 1999 |
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