Optimization of Alkaline Protease Production from Bacillus sp. by Response Surface Methodology |
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Authors: | Sumant Puri Qasim Khalil Beg Rani Gupta |
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Institution: | (1) Department of Microbiology, University of Delhi South Campus, Benito Juarez Marg, New Delhi 110 021, India, IN |
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Abstract: | High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and
inoculum density, suggested inoculum density to be an insignificant variable. However, incubation time had a profound effect
on protease yields at all the concentrations of carbon and nitrogen used. The response surface raised and flattened with increase
in time of incubation, and maximum protease production up to 1939 U/ml was obtained after 96 h of incubation. The model equation
obtained was validated experimentally at maximum starch (15 mg/ml) and peptone (7.5 mg/ml) concentration with increased incubation
time up to 144 h in the presence of minimum inoculum density (1%). An overall 2.6-fold increase in protease production was
obtained as compared with mean observed response (750 U/ml) at zero level of all variables.
Received: 19 July 2001 / Accepted: 15 August 2001 |
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