Degradation Kinetics of Caffeine and Related Methylxanthines by Induced Cells of <Emphasis Type="Italic">Pseudomonas</Emphasis> sp. |
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Authors: | Swati Sucharita Dash Sathyanarayana N Gummadi |
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Institution: | (1) Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, 600 036, India |
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Abstract: | In this study, the kinetics of degradation of caffeine and related methylxanthines by induced cells of Pseudomonas sp. was performed. The kinetics data showed that degradation of caffeine, theobromine, and 7-methylxanthine followed Michealis–Menten
kinetics. The values of K
m
are low for caffeine and 7-methylxanthine and high for theobromine. Degradation of caffeine and theobromine was enhanced
in the presence of NADH and NADPH, whereas the degradation of 7-methylxanthine was unaffected. Among the various metal ions
tested, Fe2+ was found to enhance the rate of degradation for all three substrates, whereas Zn2+ and Cu2+ inhibited the degradation of caffeine and theobromine but not 7-methylxanthine. The differences in kinetic parameters and
cofactor requirement suggest the possibility of the involvement of more than one N-demethylases in the caffeine catabolic
pathway in Pseudomonas sp. The induced cells can serve as effective biocatalysts for the development of biodecaffeination techniques. |
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Keywords: | |
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