Expression of Intracellular Enzymes During Hyphal Aggregate Formation in a Fruiting-Impaired Variant of Agaricus bisporus |
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Authors: | John BW Hammond Kerry S Burton |
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Institution: | (1) Faculty of Science and Technology, NESCOT, Reigate Road, Ewell, Surrey KT17 3DS, UK , GB;(2) Department of Microbial Biotechnology, Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK , GB |
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Abstract: | The specific activity and enzyme protein concentration of the developmentally regulated enzyme glucose 6-phosphate dehydrogenase
(G6PD) were measured in the developing aggregates and supporting mycelium of a fruiting-impaired variant strain of Agaricus bisporus. The nonregulated enzymes mannitol dehydrogenase (MD) and hexokinase (HK) were assayed for comparison. G6PD activity was
higher in aggregates than in the mycelium, whereas MD and HK activities varied little between mycelium and aggregates. Enzyme
protein levels varied in a way different from enzyme activity, suggesting the presence of inactive enzyme at times during
development. The raised level of G6PD in aggregates provides a possible mechanism for the increased mannitol concentration
previously observed in aggregates. There was no parallel to the rapid increase in G6PD activity associated with primordium
development of normally fruiting strains growing on compost. |
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Keywords: | |
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