Role of the all1549 (ana-rsh) gene, a relA/spoT homolog, of the Cyanobacterium Anabaena sp. PCC7120 |
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| Authors: | Ning Degang Qian Yaru Miao Xiaogang Wen Chongwei |
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| Institution: | (1) Department of Environment Sciences, College of Environment, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, China;(2) Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, China;(3) Department of Pharmacy, College of Pharmacy, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, China |
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| Abstract: | The role of a single relA/spoT homolog all1549 (designated hereafter as ana-rsh) of the cyanobacterium Anabaena sp. PCC7120 was investigated. The complementation test in Escherichia
coli showed that the protein encoded by ana-rsh possesses guanosine tetraphosphate (p)ppGpp-synthase/hydrolase activity. Under laboratory growth conditions, a low level
of ppGpp was detected in Anabaena sp. PCC7120 and the loss of ana-rsh was lethal. Amino acid starvation induced ppGpp accumulation to an appropriate level, and nitrogen deficiency did not alter
the ppGpp concentration in Anabaena cells. These data suggest that ana-rsh is required for cell viability under normal growth conditions and involved in the (p)ppGpp-related stringent response to
amino acid deprivation, but not related to heterocyst formation and nitrogen fixation of Anabaena sp. PCC7120. |
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