Study of intracellular localization of the proteolytic enzyme complex and its protein inhibitors in bombyx grain |
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Authors: | D V Yarygin N O Min’kova Yu B Filippovich |
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Institution: | 1. Sholokhov Moscow State Humanitarian University, 109240, Moscow, Russia 2. Moscow State Pedagogical University, 129278, Moscow, Russia
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Abstract: | Intracellular localization of serine, cysteine and aspartate proteases, as well as their protein inhibitors, in bombyx grain in the postdiapause period of embryogenesis has been studied. Proteolytic activity of aspartate and cysteine proteases was found in lysosomal, mitochondrial, and nuclear fractions of grains. Serine protease activity was not observed in subcellular fractions of grains of the fourth day of postdiapause development. It has been shown that activities of protein inhibitors and certain peptide hydrolases in subcellular fractions provide consistent functioning and fine regulation of the proteolytic enzyme complex. |
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