重组反义GFAP逆转录病毒对星形胶质细胞在胶质瘢痕增生中作用的研究

本文研究了脑穿刺损伤后伤灶组织中大胶质细胞的变化、性激素对脑损伤后星形胶质细胞反应的影响,以及反义胶质原纤维酸性蛋白（ＧＦＡＰ）逆转录病毒表达载体对Ａｓｔ形态结构,反应性胶质化及胶质瘢痕形成的作用。结果表明,胶质瘢痕中增生的大胶质细胞主要是Ａｓｔ,ＧＦＡＰ对维持Ａｓｔ的形态结构及功能具有重要作用;少突胶质细胞在胶质瘢痕形成过程中不是反应活跃的细胞成分;性激素对Ａｓｔ的反应性胶质化有一定程度的抑制作

Studies on the effects of astrocyte during glial scar formation by recombinant antisense GFAP retrovirus

The present study was to investigate the changes of macroglia and the effect of gonadal hormone on reactive gliosis and of antisense GFAP retrovirus on astrocyte,gliosis and glial scar formation after brain stab injury (BSI). The results indicate that astrocytes are the main cells of glial scar. GFAP plays an important role in the maintenance of structure and function of astrocytes. The oligodendrocyte is not an active cell during glial scar formation. The gonadal hormone can modify the reactivity of astrocyte after BSI, but has no significant effect on differentiation and proliferation of astrocyte. The recombined GFAP retrovirus can effectively inhibit the growth of astrocyte, and GFAP expression of injured astrocyte in vitro and glial scar forming in vivo.