针刺抗脑缺血性神经元凋亡作用与机制的研究

本研究在肯定针刺抗缺血性神经元凋亡的基础上,进一步探讨针刺对内源性促凋亡因素及抑制凋亡因素的调整作用,寻找针刺对元保护作用的切入点及作用机制。结果表明：（１）针刺能减轻脑缺血导致的神经缺损行为异常,缩小梗塞面积;（２）ＰＩ荧光染色及ＴＵＮＥＬ染以显示：大鼠缺血皮层梗塞区有大量凋亡阳性信号细胞,电针后细胞凋亡受到明显抑制测定ＮＯ含量及观察ｃＮＯＳ和ｉＮＯＳ免疫活性显示：大鼠脑缺血－再灌注后脑内ＮＯ水

A study on the effect and mechanism of acupuncture suppression of neuronal apoptosis following cerebral ischemia

On the basis of affirming that acupuncture suppresses neuronal apoptosis following cerebral ischemia, this study investigates the mechanism of acupuncture which modulates the intrinsic factors suppressing and promoting apoptosis. The main results showed that:(1) the symptom of neral defect induced by cerebral ischemia was improved and the area of infarction following cerebra ischemia was reduced after acupuncture treatment; (2) by employing PI and TUNEL staining, the apoptosis neurons were observed following cerebral ischemia, and acupuncture was able to protect against neuronal apoptosis following cerebral ischemia; (3) using a kinetic cadmium reduction method for the determination of nitric oxide (NO) and an immunohistochemistry method for observation of NOS immunoactivity in rat brain, it was found that the content of NO in the side of infarction was higher than that of control, and the increase of NO content was positively related to iNOS immunoactivity; acupuncture could inhibit iNOS activity thereby decreasing the NO level; (4) in situ hybridization study revealed that excitotoxicity of glutamate following cerebral ischemia was mediated by NMDAR1mRNA overexpression, that was down regulated by acupuncture; (5) immunohistochemistry study revealed that the expression of nerve growth factor receptor(trk A) was induced by acupuncture after cerebral ischemia, which may play a role in antagonizing apoptosis.