| 锁核酸研究进展 |
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| 作者姓名： | Li SM Xu X Liang HP Li L |
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| 作者单位： | 1. 第三军医大学大坪医院野战外科研究所一室,重庆,400042;武警青海总队医院外科,西宁,810000
2. 第三军医大学大坪医院野战外科研究所一室,重庆,400042 |
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| 基金项目： | 国家重点基础研究发展规划项目(G19990 5 4 2 0 3),国家自然科学基金( 30 170 36 7),全军“十五”课题基金( 2 0 0 1～ 2 0 0 5 )资助课题 |
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| 摘 要： | 锁核酸(locked nucleic acid,LNA)是一种新型的寡核酸衍生物,结构中β-D-呋喃核糖的2’-O,4’-C位通过缩水作用形成环形的氧亚甲基桥、硫亚甲基桥或胺亚甲基桥,呋喃糖的结构锁定在C3’内型的N构型,形成了刚性的缩合结构。LNA作为一种新的反义核酸,具有与DNA／RNA强大的杂交亲和力、反义活性、抗核酸酶能力、水溶性好及体内无毒性等优点。LNA在基因诊断和基因治疗上有很多优势,如：单链核酸的多态性基因分型、LNA寡聚体具有高效抑制端粒酶活性及LNA修饰的DNA核酶(LNAzymes)高效清除高级结构的RNA等,有良好的应用研究前景。
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| 关 键 词： | 锁核酸 核苷酸衍生物 寡聚核苷酸 基因诊断 |
Progress in locked nucleic acid research |
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| Li SM,Xu X,Liang HP,Li L.Progress in locked nucleic acid research[J].Progress in Physiological Sciences,2003,34(4):319-323. |
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| Authors: | Li Sheng-Mao Xu Xiang Liang Hua-Ping Li Lei |
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| Institution: | Researsh Institute of Surgery, Third Military Medical University, Chongqing 400042. |
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| Abstract: | Locked nucleic acid (LNA) is a novel oligonucleotide analogue in which 2' O and 4' C positions in the b D ribofuranosyl ring are joined via an O methylene, S methylene or amino methylene moiety, locked in a C3' endol 3E north (N) type furanose conformation. LNAs posses many properties ,such as extraordinarily high hybridize affinities for complementary DNA/RNA sequences, remarkable antisense activity, nuclease resistance, good aqueous solubility and none detactable toxicity, et al. LNAs is a most promising molecule for development of diagnostics and therapeutics. For example, apply LNAs to single nucleotide polymorphisms genotyping,telomerase activity have been efficiently suppressed by LNA oligomers, efficient cleavage of highly structured RNA has been achieved using LNA modified DNAzymes (LNAzymes), and so on. |
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| Keywords: | Locked nucleic acid Nucleotide derivative Antisense oligonucleotide |
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