Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp. OU01 |
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Authors: | Sun Yuying Han Baoqin Liu Wanshun Zhang Jiquan Gao Xingshuang |
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Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China. |
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Abstract: | The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacterium sp. OU01 in shake-flask. A significant influence of (NH(4))(2)SO(4), MgSO(4).7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0g/L (NH(4))(2)SO(4), 1.3g/L MgSO(4) and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6U/mL to 118U/mL. |
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