<Emphasis Type="Italic">Desulfurispirillum alkaliphilum</Emphasis> gen. nov. sp. nov., a novel obligately anaerobic sulfur- and dissimilatory nitrate-reducing bacterium from a full-scale sulfide-removing bioreactor |
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Authors: | D Yu Sorokin M Foti B J Tindall G Muyzer |
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Institution: | (1) Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia;(2) Environmental Biotechnology Group, Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands;(3) DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7B, Mascheroder Weg 1b, 38124 Braunschweig, Germany |
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Abstract: | Strain SR 1T was isolated under anaerobic conditions using elemental sulfur as electron acceptor and acetate as carbon and energy source
from the Thiopaq bioreactor in Eerbeek (The Netherlands), which is removing H2S from biogas by oxidation to elemental sulfur under oxygen-limiting and moderately haloalkaline conditions. The bacterium
is obligately anaerobic, using elemental sulfur, nitrate and fumarate as electron acceptors. Elemental sulfur is reduced to
sulfide through intermediate polysulfide, while nitrate is dissimilatory reduced to ammonium. Furthermore, in the presence
of nitrate, strain SR 1T was able to oxidize limited amounts of sulfide to elemental sulfur during anaerobic growth with acetate. The new isolate
is mesophilic and belongs to moderate haloalkaliphiles, with a pH range for growth (on acetate and nitrate) from 7.5 to 10.25
(optimum 9.0), and a salt range from 0.1 to 2.5 M Na+ (optimum 0.4 M). According to phylogenetic analysis, SR 1T is a member of a deep bacterial lineage, distantly related to Chrysiogenes arsenatis (Macy et al. 1996). On the basis of the phenotypic and genetic data, the novel isolate is placed into a new genus and species, Desulfurispirillum alkaliphilum (type strain SRT = DSM 18275 = UNIQEM U250).
Nucleotide sequence accession number: the GenBank/EMBL accession number of the 16S rRNA gene sequence of strain SR 1T is DQ666683. |
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Keywords: | Desulfurispirillum alkaliphilum Sulfur-reducing Dissimilatory nitrate-reducing Haloalkaliphilic |
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