Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively |
| |
Authors: | Yun-Zhuang Mou Xing-Xing Qiu Mei-Lin Zhao Heng-Lin Cui Dickson Oh Mike L Dyall-Smith |
| |
Institution: | 1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China 2. 5/9 Waterman Terrace, Mitchell Park, Adelaide, SA, 5043, Australia 3. School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW, 2678, Australia
|
| |
Abstract: | Two halophilic archaeal strains, R30T and tADLT, were isolated from an aquaculture farm in Dailing, China, and from Deep Lake, Antarctica, respectively. Both have rod-shaped cells that lyse in distilled water, stain Gram-negative and form red-pigmented colonies. They are neutrophilic, require >120?g/l NaCl and 48–67?g/l MgCl2 for growth but differ in their optimum growth temperatures (30?°C, tADLT vs. 40?°C, R30T). The major polar lipids were typical for members of the Archaea but also included a major glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The 16S rRNA gene sequences of the two strains are 97.4?% identical, show most similarity to genes of the family Halobacteriaceae, and cluster together as a distinct clade in phylogenetic tree reconstructions. The rpoB′ gene similarity between strains R30T and tADLT is 92.9?% and less to other halobacteria. Their DNA G?+?C contents are 62.4–62.9?mol?% but DNA–DNA hybridization gives a relatedness of only 44?%. Based on phenotypic, chemotaxonomic and phylogenetic properties, we describe two new species of a novel genus, represented by strain R30T (=?CGMCC 1.10593T?=?JCM 17270T) and strain tADLT (=?JCM 15066T?=?DSMZ 22187T) for which we propose the names Halohasta litorea gen. nov., sp. nov. and Halohasta litchfieldiae sp. nov., respectively. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|