Multiple origins of European populations of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), a liver parasite of ruminants |
| |
| Authors: | Králová-Hromadová Ivica Bazsalovicsová Eva Stefka Jan Spakulová Marta Vávrová Silvia Szemes Tomá? Tkach Vasyl Trudgett Alan Pybus Margo |
| |
| Institution: | 1. Parasitological Institute, Slovak Academy of Sciences, Ko?ice, Slovakia;2. Biology Centre ASCR, Institute of Parasitology and Faculty of Science, University of South Bohemia, ?eské Budějovice, Czech Republic;3. Entomology Department, Natural History Museum, London, UK;4. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia;5. Department of Biology, University of North Dakota, Grand Forks, ND, USA;6. School of Biological Sciences, The Queen’s University of Belfast, Belfast, UK;7. Alberta Fish and Wildlife Division and Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada;1. Department of Animal Pathology (INVESAGA Group), Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain;2. Department of Animal Health, Faculty of Veterinary Sciences, University of León, 24071 León, Spain;1. University of Bristol, School of Biological Sciences and School of Veterinary Science, Bristol Life Sciences Building, 24, Tyndall Avenue, Bristol BS8 1TQ, United Kingdom;2. University of Pretoria, Faculty of Veterinary Science, Private Bag X20, Hatfield 0028, South Africa;1. Departamento de Biologia e CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal;2. Instituto de Investigación en Recursos Cinegéticos (UCLM-CSIC-JCCM), Ronda de Toledo 12, 13071 Ciudad Real, Spain |
| |
| Abstract: | The giant liver fluke, Fascioloides magna, a liver parasite of free-living and domestic ruminants of Europe and North America, was analysed in order to determine the origin of European populations and to reveal the biogeography of this originally North American parasite on the European continent. The variable fragments of the mitochondrial cytochrome c oxidase subunit I (cox1; 384bp) and nicotinamide dehydrogenase subunit I (nad1; 405bp) were used. Phylogenetic trees and haplotype networks were constructed and the level of genetic structuring was evaluated using population genetic tools. In F. magna individuals originating from all European foci of infection (Italy, Czech Republic and Danube floodplain forests involving the territories of Slovakia, Hungary and Croatia) and from four of five major North American enzootic areas, 16 cox1 and 18 nad1 haplotypes were determined. The concatenated sequence set produced 22 distinct haplotypes. The European fluke populations were less diverse than those from North America in that they contained proportionately fewer haplotypes (eight), while a more substantial level of genetic diversity and a greater number of haplotypes (15) were recorded in North America. Only one haplotype was shared between the European (Italy) and North American (USA/Oregon and Canada/Alberta) flukes, supporting a western North American origin of the Italian F. magna population. Haplotypes found in Italy were distinct from those determined in the remaining European localities which indicates that introduction of F. magna to the European continent occurred more than once. In the Czech focus of infection, a south-eastern USA origin was revealed. Identical haplotypes, common to parasites from the Czech Republic and from an expanding focus in Danube floodplain forests, implies that the introduction of F. magna to the Danube region came from an already established Czech focus of infection. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
