Foetal and adult cardiomyocyte progenitor cells have different developmental potential |
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Authors: | Patrick Van Vliet Anke M Smits Teun P De Boer Tom H Korfage Corina HG Metz Marta Roccio Marcel AG Van Der Heyden Toon AB Van Veen Joost PG Sluijter Pieter A Doevendans Marie‐José Goumans |
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Institution: | 1. Department of Cardiology, Division Heart & Lungs, University Medical Center Utrecht, Utrecht, The Netherlands;2. Interuniversity Cardiology Institute Netherlands (ICIN), Utrecht, The Netherlands;3. Present address: Department of Anatomy & Embryology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands.;4. Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands;5. Department of Medical Physiology, Division Heart & Lungs, University Medical Center Utrecht, Utrecht, The Netherlands |
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Abstract: | In the past years, cardiovascular progenitor cells have been isolated from the human heart and characterized. Up to date, no studies have been reported in which the developmental potential of foetal and adult cardiovascular progenitors was tested simultaneously. However, intrinsic differences will likely affect interpretations regarding progenitor cell potential and application for regenerative medicine. Here we report a direct comparison between human foetal and adult heart‐derived cardiomyocyte progenitor cells (CMPCs). We show that foetal and adult CMPCs have distinct preferences to differentiate into mesodermal lineages. Under pro‐angiogenic conditions, foetal CMPCs form more endothelial but less smooth muscle cells than adult CMPCs. Foetal CMPCs can also develop towards adipocytes, whereas neither foetal nor adult CMPCs show significant osteogenic differentiation. Interestingly, although both cell types differentiate into heart muscle cells, adult CMPCs give rise to electrophysiologically more mature cardiomyocytes than foetal CMPCs. Taken together, foetal CMPCs are suitable for molecular cell biology and developmental studies. The potential of adult CMPCs to form mature cardiomyocytes and smooth muscle cells may be essential for cardiac repair after transplantation into the injured heart. |
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Keywords: | cardiac progenitor cell foetal adult multipotency differentiation |
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