|
|||||
|
|
Upregulation of MAPKAPK5-AS1, PXN-AS1 and URB1-AS1 lncRNAs in non-functioning pituitary adenoma |
|
| Authors: | Mohammad Taheri Arash Safarzadeh Arefe Bahranian Solat Eslami Nader Akbari Dilmaghani Soudeh Ghafouri-Fard Guive Sharifi |
| Institution: | 1. Institute of Human Genetics, Jena University Hospital, Jena, Germany;2. Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Contribution: Investigation (equal), Methodology (equal);3. Skull Base Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Contribution: Investigation (equal), Methodology (equal);4. Department of Medical Biotechnology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran Contribution: Formal analysis (equal), Visualization (equal);5. Skull Base Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Contribution: Investigation (equal), Validation (equal);6. Men's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;7. Skull Base Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
| Abstract: | Long non-coding RNAs (lncRNAs) have been shown to be dysregulated in a variety of malignant and non-malignant lesions including non-functioning pituitary adenomas (NFPAs). In the current experimental study, we have selected six lncRNAs, namely MAPKAPK5-AS1, NUTM2B-AS1, ST7-AS1, LIFR-AS1, PXN-AS1 and URB1-AS1 to assess their expression in a cohort of Iranian patients with NFPA. MAPKAPK5-AS1, PXN-AS1 and URB1-AS1 were shown to be over-expressed in NFPA tissues compared with control samples (Expression ratios (95% CI) = 10 (3.94–25.36), 11.22 (4.3–28.8) and 9.33 (4.12–21.12); p values < 0.0001, respectively). The depicted ROC curves showed the AUC values of 0.73, 0.80 and 0.73 for MAPKAPK5-AS1, PXN-AS1 and URB1-AS1, respectively. Relative expression level of PXN-AS1 was associated with tumour subtype (p value = 0.49). Besides, relative expression levels of MAPKAPK5-AS1 and LIFR-AS1 were associated with gender of patients (p values = 0.043 and 0.01, respectively). Cumulatively, the current study indicates the possible role of MAPKAPK5-AS1, PXN-AS1 and URB1-AS1 lncRNAs in the pathogenesis of NFPAs. |
| Keywords: | lncRNA MAPKAPK5-AS1 non-functioning pituitary adenoma PXN-AS1 URB1-AS1 |