滇牡丹遗传多样性的ISSR分析

应用ISSR标记对中国西南地区特有植物滇牡丹(Paeonia delavayi)的遗传多样性进行了研究。从100个引物中筛选出10个用于正式扩增，在取自16个自然居群和1个迁地保护居群的511个个体中，检测到92个多态位点。在居群水平上，多态位点百分率（PPB）为44.61%，Nei′s基因多样性指数（H）和Shannon信息指数（I）分别为0.1657和0.2448。在物种水平上，多态位点百分率（PPB）为79.31%，Nei′s基因多样性指数（H）和Shannon信息指数（I）分别为0.2947和0.4355。居群间的遗传分化系数(G_ST)达0.4349。结果表明：滇牡丹遗传多样性水平较高，居群间遗传分化较大。结合以前的研究结果，对滇牡丹的现状进行评估的结果显示，滇牡丹并不濒危。

Genetic diversity of Paeonia delavayi as revealed by ISSRs

The genetic diversity of Paeonia delavayi , a species endemic to Southwest China, was analyzed using inter simple sequence repeat markers (ISSR). A total of 116 bands were amplified from 16 natural populations and one ex situ conserved population by 10 informative and reliable primers of 100 pre screened primers. Of them, 92 ISSR loci were polymorphic. A relatively high level of intraspecific genetic diversity was revealed: PPB = 44.61%, H = 0.1657 and I = 0.2448 at population level; PPB = 79.31%, H = 0.2947 and I = 0.4355 at species level. The G ST = 0.4349 indicated a high degree of genetic differentiation occurred among P. delavayi populations. The results of genetic diversity study, combined with field survey and previous investigations, suggest that P. delavayi is not endangered.