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应用ISSR-PCR分析蒙古栎种群的遗传多样性
引用本文:张杰,吴迪,汪春蕾,屈红军,邹学忠,杨传平.应用ISSR-PCR分析蒙古栎种群的遗传多样性[J].生物多样性,2007,15(3):292-299.
作者姓名:张杰  吴迪  汪春蕾  屈红军  邹学忠  杨传平
作者单位:1. 东北林业大学生命科学学院,哈尔滨,150040
2. 东北林业大学林学院,哈尔滨,150040
3. 辽宁省林业职业技术学院,沈阳,110101
基金项目:致谢:张富民博士为本文提供分析软件,特此致谢!
摘    要:本研究应用ISSR标记技术对东北地区的优势树种蒙古栎(Quercus mongolica)的25个种群遗传多样性进行了分析,目的是为蒙古栎早期选择提供依据。从60条ISSR引物中筛选出10个特异性强、稳定性好的引物进行ISSR分析。共获得位点数71个,其中多态位点数56个,多态位点百分率为78.87%。PopGene分析结果表明:种群的平均多态位点百分率为45.2%,Shannon表型多样性指数(I)平均值为0.25,具有较高的遗传多样性,种群间存在一定程度的基因流(^0为1.3818)和遗传分化(Nei’s信息多样性指数平均值为0.1068,Gst平均值为0.2657),种群内的基因多样度占总种群的73.43%,种群间占26.57%,表明蒙古栎种群的变异主要来源于种群内。结合聚类分析和地理变异规律把种群划分为两个大的种群组:小兴安岭种群组和长白山种群组。以上结果可为栎属种质资源的保护和利用以及物种分化研究提供基础资料。

关 键 词:遗传多样性  遗传结构
收稿时间:2006-06-27
修稿时间:2006-06-272006-10-17

Genetic diversity analysis of Quercus mongolica populations with Inter-Simple Sequence Repeats (ISSR) technique
Jie Zhang,Di Wu,Chunlei Wang,Hongjun Qu,Xuezhong Zou,Chuanping Yang.Genetic diversity analysis of Quercus mongolica populations with Inter-Simple Sequence Repeats (ISSR) technique[J].Biodiversity Science,2007,15(3):292-299.
Authors:Jie Zhang  Di Wu  Chunlei Wang  Hongjun Qu  Xuezhong Zou  Chuanping Yang
Institution:1. College of Life Sciences, Northeast Forestry University, Harbin 150040 ;2. College of Forestry, Northeast Forestry University, Harbin 150040; 3. Liaoning Forestry Technological College, Shen yang 110101
Abstract:Genetic diversity of twenty-five populations of Quercus mongolica,which is a dominant tree species from Northeast China,was investigated by the Inter-Simple Sequence Repeat(ISSR) technique,us-ing ten specific and stable primers selected from 60 primers.A total of 71 sites were generated,56 of which(78.87%) were polymorphic.The data were analyzed by PopGene.The average percentage of polymorphic loci of populations was 45.2% and demonstrated high genetic diversity(the average value of Shannon index(I) was 0.25).To some extent,both gene flow and genetic diversity were present among populations(Nm was 1.3818,average Nei's index was 0.1068,and average Gst was 0.2657).The gene proportion of diversity reached 73.43% within population but was 26.57% among populations.This demonstrated that genetic varia-tion of Q.mongolica populations was mainly within populations.According to cluster analysis and the law of geographic variation,the populations were classified into two large groups:the Xiaoxinganling group and the Changbaishan group.These results supplement information for protection and utilization of Q.mongolica,and also provide further data for the study of genetic variation and species differentiation of Quercus.
Keywords:Quercus mongolica  ISSR
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