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| 石油降解细菌的表型特性和系统发育分析 | |
| 引用本文： | 韩如晻,闵航,程志强,柳良好.石油降解细菌的表型特性和系统发育分析[J].生物多样性,2002,10(2):202-207. |
| 作者姓名： | 韩如晻 闵航 程志强 柳良好 |
| 作者单位： | 1. 浙江大学生命科学学院,杭州,310029 2. 浙江大学生物技术研究所,杭州,310029 3. 杭州娃哈哈集团有限公司,杭州,310020 |
| 基金项目： | 国家自然科学基金（30170030） |
| 摘 要： | 从3种不同土壤中分离和纯化得到10个石油降解细菌菌株，分离菌株均为好氧菌、革兰氏阴性菌、不形成芽孢的杆菌，10个菌株均能利用中等链长的烷烃、柴油和原油作为碳源，而不能以单环芳烃和多环芳烃为碳源。根据其生理生化性状和16SrDNA序列分析结果表明，分离菌株EVA5，EVA6，EVA7，EVA8和EVA9为假单胞菌（Pseudomonas spp.），EVA10、EVA11、EVA12、EVA13和EVA14为不动杆菌（Acinetobacter spp.），均属于Proteobac-teria的γ亚群。 |
| 关 键 词： | 石油降解细菌 表型特性 系统发育分析 |
| 文章编号： | 1005-0094(2002)02-0202-06 |
| 收稿时间： | 2001-7-16 |
| 修稿时间： | 2001年7月16日 |
Phenotypic characteristics and phylogenetic analysis of crude oil degrading bacteria |
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| HAN Ru-Yang,MIN Hang,CHENG Zhi-Qiang,LIU Liang-Hao College of Life Science,Zhejiang University,Hangzhou Institute of Biotechnology Research,Zhejiang University,Hangzhou Hangzhou Wahaha Croup Ltd.,Hangzhou.Phenotypic characteristics and phylogenetic analysis of crude oil degrading bacteria[J].Biodiversity Science,2002,10(2):202-207. | |
| Authors: | HAN Ru-Yang MIN Hang CHENG Zhi-Qiang LIU Liang-Hao College of Life Science Zhejiang University Hangzhou Institute of Biotechnology Research Zhejiang University Hangzhou Hangzhou Wahaha Croup Ltd Hangzhou |
| Institution: | HAN Ru-Yang1,MIN Hang1,CHENG Zhi-Qiang2,LIU Liang-Hao31 College of Life Science,Zhejiang University,Hangzhou 3100292 Institute of Biotechnology Research,Zhejiang University,Hangzhou 3100293 Hangzhou Wahaha Croup Ltd.,Hangzhou 310020 |
| Abstract: | Ten strains of crude oil degrading bacteria were isolated from three soil samples. The isolateswere Gram-negative, aerobic, non-spore-forming rods. All of the ten isolates were able to grow on n-al-kanes with medium length, diesel fuel and crude oil, but not polycyclic aromatic hydrocarbon. Based onphenotypic characteristics and 16S rDNA sequence analysis, ten isolates were identified as Pseudomonasspp. (strain EVA5, EVA6, EVA7, EVA8, EVA9 ) and Acinetobacter spp. (strain EVA10, EVA11,EVA12, EVA13, EVA14 ), respectively. |
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