Somatic embryogenesis of two new <Emphasis Type="Italic">Panax ginseng</Emphasis> cultivars,Yun-Poong and Chun-Poong |
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Authors: | Y J Kim M K Kim J S Shim R K Pulla D C Yang |
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Institution: | (1) Korea Ginseng and Tobacco Research Institute, Taejeon, 305-345, Korea |
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Abstract: | Somatic embryogenesis from single cells is important for normal plant regeneration of ginseng. Cotyledon explants from zygotic
embryos of two new ginseng cultivars, Chun-Poong and Yun-Poong, produced somatic embryos on Murashige and Skoog (MS) basal
medium and MS medium containing growth regulators. The highest frequency of single somatic embryo formation was obtained when
cotyledon explants were excised from premature (cultured for 1 day) zygotic embryos (about 6 mm in length) of both cvs. Chun-Poong
and Yun-Poong and then cultured on MS medium supplemented with 7% sucrose. The frequency of single somatic embryo formation
was strongly enhanced when Chun-Poong cotyledons were subjected to plasmolysis with 0.1–0.5 M sucrose for 24 h and Yun-Poong
cotyledons to plasmolysis with 1.0 M sucrose for 24 h and then cultured on MS medium with 2,4-D. |
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