Comparative study on calli from two reed ecotypes under heat stress |
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Authors: | L Song Y Jiang H Zhao Zh Zhang |
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Institution: | 1.School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai,P.R. China;2.State-Owned Miaoshou Forest Farm,Jingde Anhui,P.R. China |
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Abstract: | The different physiological responses to heat stress in calli from two ecotypes of common reed (Phragmites communis Trin.) plants (dune reed (DR) and swamp reed (SR)) were studied. The relative water content, the relative growth rate, cell
viability, membrane permeability (MP), H2O2 content, MDA content, proline level, and the activities of enzymes, such as superoxide dismutase (SOD), catalase (CAT), peroxidase
(POD), ascorbate peroxidase (APX), glutathione reductase (GR), and lipoxygenase (LOX) were assayed. Results showed that under
heat stress, DR callus could maintain the higher relative growth rate and cell viability than SR callus, while H2O2 content, MDA content, and MP in SR callus increased more than in DR callus. The activities of antioxidant enzymes, such as
SOD, CAT, POD, APX, and GR in two calli were enhanced by high temperature. However, antioxidant enzymes in DR callus showed
the higher thermal stability than those in SR callus. LOX activity increased more in SR callus than in DR callus under heat
stress. High temperature markedly elevated proline content in DR callus whereas had no effect on that in SR callus. Taken
together, DR callus is more thermotolerant than SR callus, which might be due to the higher activity of antioxidant enzymes
and proline level compared with SR callus under heat stress. |
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