Repeated fed-batch cultivation of Arthrospira (Spirulina) platensis using urea as nitrogen source |
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| Authors: | Marcelo C Matsudo Raquel P Bezerra Sunao Sato Patrizia Perego Attilio Converti João Carlos M Carvalho |
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| Institution: | 1. Department of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes 580, Bl. 16, 05508-900 São Paulo-SP, Brazil;2. Department of Chemical and Process Engineering “G.B. Bonino”, University of Genoa, via Opera Pia 15, 16145 Genoa, Italy;1. Escola de Química, Federal University of Rio de Janeiro, Av. Horácio Macedo, 2030, Centro de Tecnologia, Bl. E, Sala 201, Rio de Janeiro, RJ 21941-909, Brazil;2. Instituto de Química, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos 149, Centro de Tecnologia, Bl. A Sala 532, Rio de Janeiro, RJ 21941-909, Brazil;1. Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande, 96203-900 Rio Grande-RS, Brazil;2. Department of Bromatological Analysis, Faculty of Pharmacy, Federal University of Bahia, 40170-115 Salvador-BA, Brazil;3. Laboratory of Microbiology and Biochemistry, College of Chemistry and Food Engineering, Federal University of Rio Grande, 96203-900 Rio Grande-RS, Brazil;4. Department of Food Science and Technology, Center for Agrarian Sciences, Federal University of Santa Catarina, 88034-000 Florianópolis-SC, Brazil;1. Laboratory of Microbiology and Biochemistry, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, Brazil;2. Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, Brazil |
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| Abstract: | Arthrospira (Spirulina) platensis (Nordstedt) Gomont was autotrophically cultivated for biomass production in repeated fed-batch process using urea as nitrogen source, with the aim of making large-scale production easier, increasing cell productivity and then reducing the production costs. It was investigated the influence of the ratio of renewed volume to total volume (R), the urea feeding time (tf) and the number of successive repeated fed-batch cycles on the maximum cell concentration (Xm), cell productivity (Px), nitrogen-to-cell conversion yield (Yx/n), maximum specific growth rate (μm) and protein content of dry biomass. The experimental results demonstrated that R = 0.80 and tf = 6 d were the best cultivation conditions, being able to simultaneously ensure, throughout the three fed-batch cycles, the highest average values of three of the five responses (Xm = 2101 ± 113 mg L?1, Px = 219 ± 13 mg L?1 d?1 and Yx/n = 10.3 ± 0.8 g g?1). |
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