Amount and time course of ingestion of plant subcellular fractions by two thrips and one reference mite species |
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| Institution: | 1. Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea;2. Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea;1. Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea;2. College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China;3. Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea;4. Division of Crop Protection, National Institute of Agricultural Science, Rural Development Administration, Republic of Korea;1. Tasmanian Institute of Agriculture, University of Tasmania, Hobart, Tasmania, Australia;2. Department of Primary Industries, Parks, Water and Environment, Kings Meadows Tasmania, Australia;1. Department of Logistics Management, Shu-Te University, No. 59, Hengshan Rd., Yanchao Dist., Kaohsiung City 82445, Taiwan, ROC;2. Department of Plant Medicine, National Pingtung University of Science and Technology, 1 Shue Fu Rd., Neipu, Pingtung 91201, Taiwan, ROC;3. Department of Entomology, National Chung Hsing University, 250 Kuo Kuang Rd., Taichung 40227, Taiwan, ROC;1. Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea;2. Department of Veterinary Animal Sciences, University of Massachusetts, Amherst, MA 01002, USA;3. Department of Entomology, Kansas State University, Manhattan, KS 66506, USA |
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| Abstract: | Thrips are known as cell-feeding sucking pests, but little information is available on which and how much of each subcellular fraction they ingest. In this study, the ingested amounts of nuclei and plastids in two representative thrips species (the western flower thrips, Frankliniella occidentalis, and the garden thrips, Frankliniella intonsa) along with two reference sucking pests (the two-spotted spider mite, Tetranychus urticae, a cell feeder, and the brown planthopper, Nilaparvata lugens, a sap feeder) were quantified by quantitative PCR using phytoene desaturase and rubisco as respective marker genes following feeding. The ingested amounts of plastids were significantly greater than those of nuclei in the thrips and mite species. In the thrips species, however, the fold differences in ingested amount between the two fractions were substantially lower than their original ratio in intact plant cells, suggesting that thrips ingest nuclei more selectively than plastids. Unlike the thrips species, the ratio between nuclei and plastids increased in T. urticae. In contrast to these cell-feeding insects, no subcellular fraction was detected in N. lugens. These findings suggest that transgenic expression of foreign hairpin RNA in the nucleus would deliver a substantial amount of target molecules to cell-feeding sucking pests, but not sap-feeding pests, when employing ingestion RNA interference-based control strategies. |
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