Knockdown of glycogen phosphorylase and glycogen synthase influences expression of chitin synthesis genes of rice brown planthopper Nilaparvata lugens |
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| Institution: | 1. School of Biological and Agricultural Science and Technology, Key Laboratory of Protection and Utilization of Animal Resource in Chishui River Basin, Zunyi Normal University, Zunyi, Guizhou 563006, China;2. College of Life and Environmental Sciences, Hangzhou Normal University, Zhejiang, Hangzhou, 310036, China;1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Agricultural College of Shanxi Agricultural University, Taigu 030801, China;1. Department of vegetable crops, Korea National College of Agriculture and Fisheries, Jeonju-si, Jeollabuk-do 54874, Republic of Korea;2. Cheorwon plasma research institute, Cheorwon-gun, Gangwon-do 24047, Republic of Korea;1. Department of Botany, University of Rajasthan, J. L. N. Marg, Jaipur 302004, Rajasthan, India;2. National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune, Maharashtra 411007, India;3. Central Muga Eri Research and Training Institute, (Central Silk Board) Lahdoigarh, Jorhat 785700, Assam, India;1. Department of Agricultural Technology, Faculty of Science and Technology, Thammasat University Rangsit Centre, Khlong Nueng, Klong Luang, Pathum Thani 12120, Thailand;2. Department of Entomology, Faculty of Agriculture, Khon Kaen University, Nai Muang, Muang, Khon Kaen 40002, Thailand |
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| Abstract: | Glycogen synthase (GS) and glycogen phosphorylase (GP) are two key enzymes in the glycogen synthesis pathway, which catalyze trehalose and glucose transformation in insects. GS and GP can be regulated by trehalose metabolism, which plays an important role in insect growth. However, it is not known whether these genes can be targeted for pest control through regulation of chitin metabolism. We studied the function of Nilaparvata lugens GS and GP (NLGS and NLGP, respectively) using RNA interference, and reported that trehalose and the chitin biosynthesis pathways are regulated by GP and GS, especially TPS3, TRE1-1, and G6PI1, which decreased following knockdown of these two genes. The expression levels of TPS1, TPS2, and several chitin synthesis pathway family genes were significantly increased following dsNlGP injection. Additionally, despite there being no apparent change to the chitin content, an abnormal molting phenotype and wing deformity appeared, and close to 25% insects died. These results demonstrate that silencing of NLGP or NLGS can lead to molting deformities and an elevated mortality rate through the regulation of chitin pathway genes and chitinase genes. NLGP may play a key role in chitin synthesis due to the number of genes regulated, and higher deformity and mortality rates resulting from its knockdown. |
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