Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli |
| |
| Institution: | 1. Department of Chemical and Biological Engineering, University of Wisconsin, Madison, United States;2. Microbiology Doctoral Training Program, University of Wisconsin, Madison, United States;1. Ministry of Education Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China;2. Center for Nano and Micro Mechanics, Tsinghua University, Beijing 100084, China;3. Beijing Key Laboratory of Protein Therapeutics, Tsinghua University, Beijing 100084, China;1. Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main St, Houston, TX 77005, USA;2. Department of Bioengineering, Rice University, 6100 Main St, Houston, TX 77005, USA;3. Department of Chemistry, University of California Davis, One Shields Avenue, Davis, CA 95616, USA;4. Biochemistry & Molecular Medicine, University of California Davis, One Shields Avenue, Davis, CA 95616, USA;5. Genome Center, University of California Davis, One Shields Avenue, Davis, CA 95616, USA |
| |
| Abstract: | Polyhydroxyalkanoates that contain the medium-chain-length monomers (mcl-PHAs) have a wide range of applications owing to their superior physical and mechanical properties. A challenge to synthesize such mcl-PHAs from unrelated and renewable sources is exploiting the efficient metabolic pathways that lead to the formation of precursor (R)-3-hydroxyacyl-CoA. Here, by engineering the reversed fatty acid β-oxidation cycle, we were able to synthesize mcl-PHAs in Escherichia coli directly from glucose. After deletion of the major thioesterases, the engineered E. coli produced 6.62 wt% of cell dry weight mcl-PHA heteropolymers. Furthermore, when a low-substrate-specificity PHA synthase from Pseudomonas stutzeri 1317 was employed, recombinant E. coli synthesized 12.10 wt% of cell dry weight scl–mcl PHA copolymers, of which 21.18 mol% was 3-hydroxybutyrate and 78.82 mol% was medium-chain-length monomers. The reversed fatty acid β-oxidation cycle offered an efficient metabolic pathway for mcl-PHA biosynthesis in E. coli and can be further optimized. |
| |
| Keywords: | Polyhydroxyalkanoates Reversed fatty acid β-oxidation cycle Metabolic engineering |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
