Continuous dielectrophoretic separation of cell mixtures |
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Authors: | H A Pohl Karan Kaler |
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Institution: | (1) Department of Physics, Oklahoma State University, 74074 Stillwater, OK |
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Abstract: | Use of stream-centered dielectrophoresis (1–4) produced continuous separations on three cell mixtures (1)Chlorella vulgaris withNetrium digitus, (2)Ankistrodesmus falcatus withStaurastrum gracile, and (3)Saccharomyces cerevisiae withNetrium digitus. Maximal separations were obtained for these mixtures of live cells at 100 kHz, 600 kHz, and 2.0 MHz, respectively. The technique
was restricted to a frequency range of 0.01–32 MHz, and to suspensions of low conductivity in which microorganisms such as
these algae and yeast are tolerant. Extension, however, to cellular organisms requiring higher osmolarity is readily feasible
through the use of nonionic solutes such as sucrose, mannose, glycine, etc. |
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Keywords: | Cell sorting by continuous dielectrophoresis dielectrophoresis continuous in cell sorting yeast cell sorting algae cell sorting |
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