Gene Expression of Neuropilin-1 and Its Receptors,VEGF/Semaphorin 3a,in Normal and Cancer Cells |
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Authors: | Ding Haixia Zhang Jingsong Jiang Lei Dong Hairong Wang Jun Xiao Hang Chen Weixian |
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Institution: | (1) Department of Geriatric Neurology, The First Affiliated Hospital of Nanjing Medical University, No.300 Guangzhou Street, Nanjing, Jiangsu Province, 210029, People’s Republic of China;(2) Department of Emergency, The First Affiliated Hospital of Nanjing Medical University, 210029 Nanjing, China;(3) Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, 210029 Nanjing, China;(4) The Ministry of Education Key Lab of Modern Toxicology of Nanjing Medical University, No.140 Hanzhong Road, Nanjing, Jiangsu Province, 210029, People’s Republic of China; |
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Abstract: | Extracellular domains of the transmembrane glycoprotein, neuropilin-1 (Np1), specifically bind an array of factors and co-receptors
including class-3 semaphorins (Sema3a), vascular endothelial growth factor (VEGF), hepatocyte growth factor, platelet-derived
growth factor BB, transforming growth factor-β 1 (TGF-β1), and fibroblast growth factor2 (FGF2). Np1 may have a role in immune
response, tumor cell growth, and angiogenesis, but its relative expression in comparison to its co-primary receptors, VEGF
and Sema3a, is not known. In this study we determined the mRNA expression of Np1 and its co-receptors, VEGF and Sema3a, and
the ratio of VEGF/Sema3a in different human and rodent cell lines. Expression of Np1, VEGF and Sema3a is very low in cells
derived from normal tissues, but these proteins are highly expressed in tumor-derived cells. Furthermore, the ratio of VEGF/Sema3a
is highly variable in different tumor cells. The elevated mRNA expression of Np1 and its putative receptors in tumor cells
suggests a role for these proteins in tumor cell migration and angiogenesis. As different tumor cells exhibit varying VEGF/Sema3a
ratios, it appears that cancer cells show differential response to angiogenic factors. These results bring to light the individual
variation among the cancer-related genes, Np1, VEGF, and Sema3a, and provide an important impetus for the possible personalized
therapeutic approaches for cancer patients. |
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