Mammalian expression of single chain variable region fragments dimerized by Fc regions |
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| Authors: | Tomohiro Kato Keiko Sato Satoshi Suzuki Hiroko Sasakawa Manae Kurokawa Kusuki Nishioka Kazuhiko Yamamoto |
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| Institution: | (1) Institute of Medical Science, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae-ku, 216 Kawasaki, Kanagawa, Japan;(2) Department of Biotechnology, Tokyo Science University, Chiba, Japan;(3) Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Itabashi, Tokyo, Japan;(4) Department of Internal Medicine II, Fukushima Medical College, Fukushima, Japan |
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| Abstract: | To promote application of a single chain variable region fragment (sFv) in immunoglobulins, a sFv gene was connected to an IgG1 Fc gene, designated as a sFvc gene, and used for transfection of Sp2/0. As a result, the sFvc protein was found to be secreted in a dimeric form. It is thus felt that the sFvc protein, which mimicks the shape of a naturally occurring antibody, can be simple and useful to reproduce divalency and Fc-associated effecter functions as seen in a natural antibody.Abbreviations Abbreviations sFv
single chain variable region fragment
- Fc
constant region of immunoglobulin
- sFvc
single chain variable region fragment with an Fc region |
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| Keywords: | Fc region single chain variable region fragment immunoglobulin |
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