Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from <Emphasis Type="Italic">Tamarix androssowii</Emphasis> |
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Authors: | Yu Cheng Wang Guan Zheng Qu Hong Yan Li Ying Jie Wu Chao Wang Gui Feng Liu Chuan Ping Yang |
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Institution: | (1) Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, 26 Hexing Road, 150040 Harbin, China;(2) Heilongjiang Academy of Forestry, 134 Haping Road, 150040 Harbin, China; |
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Abstract: | Superoxide dismutases (SODs) play important role in stress tolerance of plants. In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana × P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC)
and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that
SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants
when exposed to NaCl stress. In addition, the relative weight gains of the transgenic plants were 8- to 23-fold of those observed
for WT plants after NaCl stress for 30 days. The data showed that the SOD activities that increased in transgenic lines are
1.3–4-folds of that increased in the WT plant when exposed to NaCl stress. Our analysis showed that increases in SOD activities
as low as 0.15-fold can also significantly enhance salt tolerance in transgenic plants, suggesting an important role of increased
SOD activity in plant salt tolerance. |
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