RNA interference targeting CITRON can significantly inhibit the proliferation of hepatocellular carcinoma cells |
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Authors: | Yinghui?Fu Jian?Huang Ke-Sheng?Wang Xin?Zhang Email author" target="_blank">Ze-Guang?HanEmail author |
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Institution: | (1) National Human Genome Centre of Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Rui-Jin Road II, 200025 Shanghai, China;(2) Shanghai-MOST Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Centre in Shanghai, 351 Guo Shou-Jing Road, 201203 Shanghai, China; |
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Abstract: | CITRON (rho-interacting, serine/threonine kinase 21), which is a key component of the midbody, is essential for cytokinesis.
However, the role of CITRON in hepatocellular carcinoma (HCC) is poorly understood. Here we first measured the expression
of CITRON in HCC specimens by quantitative real-time RT–PCR and immunohistochemical staining. The results showed CITRON to
be frequently up-regulated in HCC as compared with adjacent non-tumour tissues. Then we employed adenovirus-mediated RNA interference
against CITRON to assess its anti-proliferation effect on SMMC-7721 cells, a representative HCC cell line. The resulting data
demonstrated that CITRON knockdown was capable of inhibiting the proliferation and colony formation of SMMC-7721 cells, with
an obvious increase of multinucleated cells. Furthermore, we subcutaneously injected the SMMC-7721 cells with the CITRON knockdown
into nude mice to evaluate the tumourigenicity. The data indicated that adenovirus-mediated RNA interference can suppress
tumourigenicity in vivo of HCC cells. Our data suggest that CITRON may be a potential target for therapeutic intervention
in HCC. |
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