The genetic and physiological analysis of late-flowering phenotype of T-DNA insertion mutants of AtCAL1 and AtCAL2 in Arabidopsis |
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| Authors: | Jihong Zhang Xinhong Guo Xiushan Li Feng Xiang Bo Zhou Dashi Yu Dongying Tang Xuanming Liu |
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| Institution: | (1) College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, China;(2) Department of Biology Engineering, Xiangtan University, Xiangtan, 411105, China; |
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| Abstract: | The homozygous T-DNA mutants of AtCAL1 (Rat1) and AtCAL2 (Rat2) were obtained. The double mutant of Rat2/Rat1RNAi was constructed which showed obvious late-flowering phenotype from others. The expression of various flowering-related genes
was studied among mutants and wild-type plants by quantitative RT–PCR. The double mutant plants showed the shortest root length
compared with T-DNA insertion mutants and wild type plants under red light, blue light, and white light. The double mutants
showed hypersensitivity to NaCl and ABA. However, these mutants had no effect on stomatal closure by ABA. |
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