Study on the association of COX-2 genetic polymorphisms with risk of gastric cancer in high incidence Hexi area of Gansu province in China |
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Authors: | Zhu Ke-Xiang Li Yu-Min Li Xun Zhou Wen-Ce Shan Yong Liu Tao |
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Institution: | (1) Department-2 of General Surgery, The First Hospital of Lanzhou University, 730000 Lanzhou, Gansu Province, China;(2) The Second Hospital of Lanzhou University, 730000 Lanzhou, Gansu Province, China |
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Abstract: | To investigate the possible association of polymorphisms, cyclooxygenase-2 (COX-2) promoter region −899G>C, COX-2 codon 587G>A,
with risk of gastric cancer in the high incidence Hexi area of Gansu province in China. Blood samples from 140 patients with
gastric carcinoma and 125 normal persons were collected in Hexi area of Gansu province in China. Polymorphisms of COX-2 −899G>A
and COX-2 587G>A were genotyped by PCR-TaqMan. For detection Helicobacter pylori infection, Warhin–Starry staining was used. Three kinds of polymorphisms of COX-2 −899G>C were GG, GC and CC. The frequencies
in gastric cancer patients were 72.9, 21.4 and 5.7%, and the frequencies in controls were 84.0, 12.8 and 3.2%, respectively.
COX-2 −899C carrier (GC + CC) increased risk of gastric carcinoma with an odds ratio 1.950 (95% CI: 1.067–3.586, P = 0.029). The genotype of COX-2 587G>A polymorphism were GG, GA and AA. The frequencies in patients group were 86.4, 11.4
and 2.2%, and the frequencies in controls were 89.6, 9.6 and 0.8%, respectively. There was no significant difference between
cases and controls in each genotype. Helicobacter pylori infection rate was 68.6% in patients group and 50.4% in healthy controls. Helicobacter pylori infection rate in gastric cancer patients was remarkably higher than that in normal people (OR: 2.147, 95% CI: 1.302–3.541,
P = 0.003). Stratification analysis was showed that COX-2 −899C carrier genotype with Helicobacter pylori infection was significantly higher in cases than that in healthy controls (OR: 4.000, 95% CI: 1.638–9.770). The polymorphism
of COX-2 −899G>C could be a risk factor for gastric cancer in high incidence Hexi area of Gansu Province in China. COX-2 −899C
carrier genotype and Helicobacter pylori positive infection may have a synergistic effect on gastric cancer in high incidence Hexi area of Gansu Province in China.
However, the polymorphisms of COX-2 587G>A is no association with gastric cancer in the high incidence Hexi area of Gansu
Province in China. |
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