Optimized procedure for expression and renaturation of recombinant human bone morphogenetic protein-2 at high protein concentrations |
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Authors: | Hongbin Zhang Jie Wu Yu Zhang Na Fu Jie Wang Shujin Zhao |
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Institution: | (1) Department of Medical Research, Guangzhou Liuhuaqiao Hospital, 111 Liuhua Road, Guangzhou, Guangdong Province, 510010, People’s Republic of China;(2) Institute of Huabo Biopharmaceutical, Guangzhou Liuhuaqiao Hospital, 111 Liuhua Road, Guangzhou, Guangdong Province, 510010, People’s Republic of China;(3) Center for Disease Control and Prevention of Guangdong Province, Guangzhou, Guangdong Province, People’s Republic of China |
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Abstract: | A prokaryotic expression system has been used to produce recombinant human bone morphogenetic protein-2 (rhBMP-2). However,
low rhBMP-2 yields and protein loss during purification and renaturation are the hurdles in the clinical application. Previous
studies have indicated that variables such as temperature, host cell, salt concentration, and culture time affect the final
rhBMP-2 yield. The optimization of these conditions in an Escherichia coli culture yielded 28.258 mg of rhBMP-2 per liter of culture. To reduce rhBMP-2 loss during purification and renaturation, we
performed purification before renaturation in the prokaryotic expression system instead of using the traditional renaturation-before-purification
approach. rhBMP-2 was separated on a Sephacryl S-300 HR column and eluted from a DEAE-Sepharose Fast Flow column. The collected
protein was refolded by dialysis with urea buffer, which was followed by dialysis with ultrapure water. The purified rhBMP-2
dimer significantly increased alkaline phosphatase (ALP) activity and osteogenic activity in the femoral muscle and showed
the same level of bone-forming activity as natural BMP-2. This optimized procedure for expression and renaturation of rhBMP-2
has potential clinical applications. |
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