Study of the factors affecting the extraction of soybean protein by reverse micelles |
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| Authors: | Xihong Zhao Yanmei Li Xiaowei He Nanjing Zhong Zhenbo Xu Liansheng Yang |
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| Institution: | (1) College of Light Industry and Food Sciences, South China University of Technology, 510640 Guangzhou, People’s Republic of China;(2) Guangzhou Medical College, 510182 Guangzhou, People’s Republic of China;(3) Department of Microbial Pathogenesis, Dental School, University of Maryland—Baltimore, 7th Floor South-650 W. Baltimore Street, Baltimore, MD 21201, USA; |
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| Abstract: | In this work, the forward and back extraction of soybean protein by reverse micelles was studied. The reverse micellar systems
were formed by anionic surfactant sodium bis(2-ethyl hexyl) sulfosuccinate (AOT), isooctane and KCl solution. The effects
of AOT concentration, aqueous pH, KCl concentration and phase volume ratio on the extraction efficiency of soybean protein
were tested. Suitability of reverse micelles of AOT and Triton-X-100/AOT mixture in organic solvent toluene for soybean protein
extraction was also investigated. The experimental results lead to complete forward extraction at the AOT concentration 120 mmol l−1, aqueous pH 5.5 and KCl concentration 0.8 mol l−1. The backward extraction with aqueous phase (pH 5.5) resulted in 100% extraction of soybean protein from the organic phase. |
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