Cloning and Characterization of a Root-specific Expressing Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme a Reductase from Ginkgo biloba |
| |
| Authors: | Guoan Shen Yongzhen Pang Weisheng Wu Zhihua Liao Lingxia Zhao Xiaofen Sun Kexuan Tang |
| |
| Institution: | (1) Plant Biotechnology Research Center, Shanghai Key Laboratory of Biotechnology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Institute of Systems Biology, Shanghai Jiao Tong University, Shanghai, 200030, China;(2) State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, 200433, People's Republic of China;(3) Faculty of Life Sciences, Southwest China Normal University, Chongqing, 400715, People's Republic of China |
| |
| Abstract: | 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC: 1.1.1.34) catalyzes the first committed step in mevalonic acid
(MVA) pathway for biosynthesis of isoprenoids. The full-length cDNA encoding HMGR was isolated from Ginkgo biloba for the first time (designated as GbHMGR, GenBank accession number AY741133), which contained a 1713 bp ORF encoding 571 amino acids. The GbHMGR genomic DNA sequence was also obtained, revealing GbHMGR had four exons and three introns. The deduced GbHMGR protein showed high identity to other plant HMGRs and contained two
trans-membrane domains and a catalytic domain. The three dimensional model of GbHMGR represented a typical spatial structure
of HMGRs. The Southern blot and RT-PCR assay results indicated that GbHMGR belonged to a small gene family, and expressed in a tissue-specific manner with a low level expression being only found in
root. The potential significance of GbHMGR gene was also discussed. |
| |
| Keywords: | Cloning Ginkgo biloba RACE 3-Hydroxy-3-methylglutaryl coenzyme A reductase |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
