Soluble expression and purification of Brucella cell surface protein (BCSP31) of Brucella melitensis and preparation of anti-BCSP31 monoclonal antibodies |
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| Authors: | Lei Zhang Xing An Wu Fang Lin Zhang Cui Hong An Yang Xin Sun Wen Tao Bai Zhi Kai Xu |
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| Institution: | (1) Department of Microbiology, and PLA Medical Key Library of Molecular Virology and Immunology, Fourth Military Medical University, Xi’an, 710032, People’s Republic of China;(2) Department of Pests and Brucellosis, Shaanxi Center for Disease Control and Prevention, Xi’an, People’s Republic of China; |
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| Abstract: | Brucella cell surface protein (BCSP31) is potentially useful for diagnosing brucellosis. We aimed to establish a monoclonal antibody
(MAb) against Brucella melitensis BCSP31 and to investigate its distribution in diagnosis. Soluble recombinant BCSP31 was successfully expressed and purified.
Two MAbs (1F1 and 1E5) against B. melitensis BCSP31, effective in detecting both recombinant and cellular proteins, were obtained and characterized. The MAbs did not
react with Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Mycobacterium tuberculosis, or Bacillus aeruginosus, but strongly reacted with BCSP31 and B. melitensis by ELISA and Western blot analysis. We also tested different Brucella species and brucellosis using the prepared anti-BCSP31 MAbs. BCSP31 and anti-BCSP31 MAbs may play important roles in future
research in diagnosing brucellosis. |
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