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Molecular characterization and expressional affirmation of the beta proteasome subunit cluster in rock bream immune defense
Authors:Saranya Revathy Kasthuri  Navaneethaiyer Umasuthan  Ilson Whang  Bong-Soo Lim  Hyung-Bok Jung  Myung-Joo Oh  Sung-Ju Jung  Sang-Yeob Yeo  Sung Yeon Kim  Jehee Lee
Institution:1. Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju, 690-756, Jeju Self-Governing Province, Republic of Korea
2. Fish Vaccine Development Center, Jeju National University, Jeju, 690-756, Jeju Self-Governing Province, Republic of Korea
3. Department of Aqualife Medicine, Chonnam National University, Chonnam, 550-749, Republic of Korea
4. Division of Applied Chemistry & Biotechnology, Department of Biotechnology, Hanbat National University, Daejeon, 305-719, Republic of Korea
5. Genetics & Breeding Research Center, National Fisheries Research & Development Institute, Geoje, 656-842, Republic of Korea
Abstract:Immunoproteasomes are primarily induced upon infection and formed by replacing constitutive beta subunits with inducible beta subunits which possess specific cleavage properties that aid in the release of peptides necessary for MHC class I antigen presentation. In this study, we report the molecular characterization and expression analysis of the inducible immunosubunits PSMB8, PSMB9, PSMB9-L, and PSMB10 from rock bream, Oplegnathus fasciatus. The three subunits shared common active site residues and were placed in close proximity to fish homologues in the reconstructed phylogenetic tree, in which the mammalian homologues formed separate clades, indicating a common ancestral origin. The rock bream immunosubunits possessed higher identity and similarity with the fish homologues. RbPSMB8, RbPSMB9, RbPSMB9-L, and RbPSMB10 were multi-exonic genes with 6, 6, 7 and 8 exons, respectively. These four genes were constitutively expressed in all the examined tissues. Immunostimulants such as lipopolysaccharide and poly I:C induced RbPSMB8, RbPSMB9, RbPSMB9-L, and RbPSMB10 in liver and head kidney, suggesting their possible involvement in immune defense in rock bream.
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