Induction of toll-like receptor (TLR) 2, and MyD88-dependent TLR- signaling in response to ligand stimulation and bacterial infections in the Indian major carp,mrigal (<Emphasis Type="Italic">Cirrhinus mrigala)</Emphasis> |
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Authors: | Madhubanti Basu Banikalyan Swain Bikash R Sahoo Nikhil K Maiti Mrinal Samanta |
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Institution: | Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyaganga, Bhubaneswar, 751002, Orissa, India. |
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Abstract: | Toll-like receptor 2 (TLR2) is a member of TLR family. It recognizes a wide range of bacteria and their products, and is involved
in inducing innate immune responses. In this article, we reported inductive expression of TLR2 and myeloid differentiation
primary response gene 88 (MyD88)-dependent signaling in the Indian major carp, mrigal (Cirrhinus mrigala) which is highly commercially important fish species in the Indian subcontinent. Ontogeny analysis of TLR2, MyD88 and TRAF6
(TNF receptor associated factor 6) genes by quantitative real-time PCR (qRT-PCR) revealed constitutive expression of these
genes in all embryonic developmental stages, indicating their involvement in embryonic innate immune defense system in fish.
Tissue specific expression analysis of these genes by qRT-PCR showed their wide distribution in various organs and tissues.
Highest expression of TLR2 was in gill, MyD88 in liver and TRAF6 was in kidney. Inductive expression of TLR2, MyD88 and TRAF6
genes were observed following peptidoglycan (PGN)-treatment, and Streptococcus uberis and Aeromonas hydrophila infections. Expression of interleukin (IL)-8 and TNF-α in various organs were significantly enhanced by PGN-treatment and
bacterial infections, and were closely associated with TLR2 induction. These findings together highlighted the contribution
of TLR2 in augmenting innate immunity in fish, and indicated it’s important role in immune surveillance of various organs
during pathogenic invasion. This study will enrich the information in understanding the innate immune mechanism in fish, and
will be helpful in developing preventive measures against infectious diseases in fish. |
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